Table 2.

Aerobic sterol uptake (high-copy vector)

StrainaGenotypeSterol uptakeb
463-1CWild type1
CJ2-A upc2-1 12.40
H1Bylr228c::LEU2 1
H1B (+)ylr228c::LEU2 1.95
KS93 (+)ylr228c::LEU2 upc2-1 15.82
KS94 (+)ylr228c::LEU2 upc2::URA3 2.92
  • a +, Strain was transformed with the YEp351 plasmid containing the YLR228c point mutation.

  • b Sterol uptake is represented as the fold difference compared to the wild type from an experiment performed in triplicate.