Table 1.

Strains and plasmids

Strain or plasmidRelevant characteristicsaSource or reference
S. mutans
 UA159Wt; Ems KmsJ. Ferretti, OU-ACGT
 NG8Wt; Ems KmsA. S. Bleiweis, University of Florida
 JH1005Blis+Ems Kms22
 BM71Wt; EmsKmsG. H. Bowden, University of Manitoba
 GB14Wt; Ems KmsG. V. Kulkarni, University of Toronto
 LT11Highly transformable mutant; Ems Kms49
 YD025UA159::pVA-GTFA; EmrThis study
 SMCC1NG8::pComC-KO; ComCEmr KmsThis study
 SMCD1NG8::pComD-KO; ComDEmr KmsThis study
 SMCE1NG8::pComE-KO; ComEEmr KmsThis study
E. coliXL1-BlueCloning hostStratagene
Plasmids
 pCR-ScriptPCR cloning vector; AmprStratagene
 pTV32OKPCR template for Tn917 Emr gene11
 pVA891Steptococcal integration plasmid; Emr35
 pVA8912Streptococcal integration plasmid derived from pVA891; EmrHorst Malke, Jena University, Jena, Germany
 pSUCRIpMK3 harboring the S. mutans gtfA gene5
 pVA-GTFApVA891 harboring a 2.4-kb fragment containing theS. mutans gtfA geneThis study
 pDL289E. coli-Streptococcus shuttle vector; Kmr4
 pComC-KOpCR-Script harboring an inactivated comCgene; Ampr EmrThis study
 pComD-KOpVA8912 harboring a 292-bp internal comDfragment; EmrThis study
 pComE-KOpVA8912 harboring a 462-bp internal comE fragment; EmrThis study
  • a Wt, wild type.