Table 1.

Strains used in this study

Strain no.GenBank accession no.aMutationbrpoSalleleDate of inoculation (mo/yr)
1594AF184095his-2555DIIdMutant4/1967
1595AF184103his-2555 DIIMutant4/1967
1596AF184102his-2555 DIIMutant4/1967
1669AF184101his-2550DIIR42AMutant4/1967
1670AF184100his-2550DIIR47AMutant4/1967
1674AF184099his-2550DIIR49BMutant4/1967
1704AF184104thy-316 2265AMutant8/1964
1706AF184098thy-317 2266Mutant8/1964
1747AF184097his-2555DIIMutant4/1958
1748AF184096his-2555DIIMutant4/1958
1602cNAχ3000Wild type10/1998
1597NAhis-140Wild type6/1954
1599NAhis-142Wild type6/1954
1600NAhis-143Wild type6/1954
1601NAhis-144Wild type6/1954
1657NAhis-2122 R5Wild type4/1958
1683NAhis-214Wild type4/1958
1684NAthy-273 2222Wild type8/1964
1685NAthy-269Wild type8/1964
1686NAthy-273 2222Wild type8/1964
1687NAthy-273 2222Wild type8/1964
1695NAthy-272 2211Wild type8/1964
1698NAthy-313 2262BWild type8/1964
1699NAthy-314 2263AWild type8/1964
1700NAthy-314 2263BWild type8/1964
1701NAthy-314 2263CWild type8/1964
1702NAthy-315 2264AWild type8/1964
1703NAthy-314 2264BWild type8/1964
  • a NA, no accession number.

  • b Strains with the same allele number (i.e.,his-2550 and his-2555) are replicates from the original auxotroph isolation.

  • c Wild-type strain χ3000 was generously provided by Cheryl Nickerson, Washington University.

  • d DII indicates the position of the mutation within the histidine operon (5).