Table 2.

Expression of genes involved in carbon metabolism ofB. subtilis JH642 grown under anaerobic conditionsa

GeneDescriptionInduction or repression (fold)
Nitrate respirationNitrite respirationFermentation
−Pyruvate+Pyruvate
lctE l-Lactate dehydrogenase1.712.8150.94.8
lctP l-Lactate permease1.49.2103.812.4
alsD Alpha-acetolactate decarboxylase8.216.545.111.1
pdhA Pyruvate dehydrogenase (E1 alpha subunit)(1.7)(3.3)(26.9)(1.8)
pdhB Pyruvate dehydrogenase (E1 beta subunit)(1.4)(2.8)(26.9)(1.9)
pdhC Pyruvate dehydrogenase (E2 subunit)(1.3)(2.0)(3.2)(1.4)
pdhD Pyruvate dehydrogenase (E3 subunit)(1.2)(1.5)(2.4)(1.1)
glpF Glycerol uptake facilitator(2.7)(5.7)(2.7)(2.9)
glpK Glycerol kinase(2.6)(6.9)(6.4)(4.5)
glpT Glycerol-3-phosphate permease(5.6)(5.8)(9.5)(1.6)
glpQ Glycerophosphoryl diester phosphodiesterase(7.5)(5.8)(9.6)(2.2)
  • a The concentration of pyruvate was 1% when added. The fold induction was the ratio of the dye intensity of the anaerobic sample to that of the aerobic sample. The fold repression (values in parentheses) was the ratio of the dye intensity of the aerobic sample to that of the anaerobic culture. Each data point is an average of the results of four independent experiments. A ratio of 2.0 or below was not considered a significant increase. −Pyruvate, without pyruvate; +Pyruvate, with pyruvate.