Table 1.

Strains used in this worka

StrainGenotypeReference or sourceb
FGSC26biA1 veA1FGSC
RMS011pabaA1 yA2 ΔargB::trpCΔB veA1 trpC80136
CLK20biA1 ΔcatA::argBΔA metG1 ΔcatB::argBΔB veA1Progeny from cross TRN1 × CLK15 (this work) (FGSC strain A1055 and ATCC MYA-116)
TLK61pabaA1 yA2 ΔcatC::argBΔC ΔargB::trpCΔB trpC801 veA1Obtained by transforming strain RMS011 with linear pLK20 (this work)
TLK12pabaA1 yA2 ΔargB::trpCΔB ΔcatB::argBΔB trpC801 veA123 (FGSC strain A1054 and ATCC MYA-118)
CLK35pabaA1 yA2 biA1 ΔcatC::argBΔC ΔcatA::argBΔA ΔcatB::argBΔB veA1Progeny from cross CLK20 × TLK61 (this work)
CLK14biA1 ΔcatA::argBΔA metG1 ΔcatB::argBΔB veA1Progeny from cross CLK12 × TLK12 (this work)
CLK15biA1 metG1 ΔcatB::argBΔB veA1Progeny from cross CLK12 × TLK12 (this work)
RYC17ΔargB::trpCΔB ΔcatA::argB veA1Partial genotype7
RYC16ΔargB::trpCΔB ΔcatA::argB ΔcatB::argB veA1Partial genotype 7
CLK36pabaA1 biA1 ΔcatC::argBΔC ΔcatA::argBΔA metG1 ΔcatB::argBΔB veA1Progeny from cross CLK20 × TLK61 (this work)
  • a To obtain triple catA catB catC mutants, strains CLK20 and TLK61 were crossed. Master plates containing progeny from this cross were screened for the lack of CatB, using 20 mM H2O2, as described previously (23). Of 94 strains, 37 lacked catB and used to extract genomic DNA. DNA samples were screened for catCdisruption by PCR, using oligonucleotides catC10 (5′AAGATTGGGTCGAAGCGG3′) and argB1 (5′CATAAGTCCGCCAGCAGG3′). The lack of CatA and CatB activities was confirmed by Zymogram analysis.

  • b FGSC, Fungal Genetics Stock Center.