Table 1.

Glycerol kinase specific activities in E. coli strains

StrainGenotypeaSource and/or referenceGrowth mediumbMean glycerol kinase sp act (U/mg) ± SD
JM83F ara glpR208 Δ(lac-proAB)rpsL(Strr) [φ80dlacΔ(lacZ)M15]thi Ry Young (7)LB1.9 ± 0.1
JM101 glpR208 supE thi Δ(lac-proAB) [F′traD36 proAB lacI q ZΔM15]CGSC (7)LB1.8 ± 0.1
TB1JM83 glpR208 hsdR(rK mK +)Ry Young (1)LB1.9 ± 0.1
DG1TB1 ΔglpK202 glpR208 6 LB0.3 ± 0.02
KH11DG1 glpK + glpR208 pro + DG1 × Hfr PK3LB2.5 ± 0.6
mG1.3 ± 0.5
KH62KH11glpR + malT::Tn10 KH11 × P1.MM130LB0.3 ± 0.02
KH20JM83glpR + malT::Tn10 JM83 × P1.MM130LB0.3 ± 0.02
KH21JM83 glpR208 malT::Tn10 JM83 × P1.MM130LB1.7 ± 0.6
Lin43 HfrC fadL701 glpK22 glpR2 phoA8 pit-10 relA1 sopT1 tonA22T2 R CGSC (11)LB2.4 ± 0.9
MC4100F araD139 Δ(argF-lac)U169 deoC1 flbB5301 ptsF25 rbsR relA1 rpsL150(Strr)CGSCLB0.4 ± 0.2
mG1.6 ± 0.7
TS100MC4100glpR2 Tim LarsonLB2.5 ± 0.5
KH44MC4100 ΔglpEGR::kan MC4100 × P1.NZ42LB1.7 ± 0.2
NZ42MC4100 φ(glpK::lacZ)(Hyb) ΔglpEGR::kan Tim LarsonNDc
MG1655λ rph-1 CGSCLB0.2 ± 0.1
mG1.5 ± 0.2
MM130 malT::Tn10 Mike MansonLB0.4 ± 0.02
  • a The allele number glpR208was assigned by the E. coli Genetic Stock Center (CGSC) after the DNA sequence was determined and is added here to the genotypes of the relevant strains.

  • b LB, LB medium; mG, minimal M9 salts with 1% (vol/vol) glycerol.

  • c ND, not determined.