Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Source or reference
Escherichia coli
 NovaBlueRecipient for cloning experimentsNovagen
 BL21 (DE3) pLysSOverproduction of proteins from pET-based plasmidsNovagen
Salmonella
 JR501Conversion of E. coli-derived plasmids to compatibility withSalmonella 20
 SJW1103Wild-type strain for motility and chemotaxis 23
 SJW1383 flhB 17
 MY2701 flhB extragenic suppressor offliK polyhook mutant 22
 MY2714 flhB extragenic suppressor offliK polyhook mutant 22
 MY3018 flhB extragenic suppressor offliK polyhook mutant 22
 MY3201 flhB extragenic suppressor offliK polyhook mutant 22
 MY3501 flhB extragenic suppressor offliK polyhook mutant 22
 MY3519 flhB extragenic suppressor offliK polyhook mutant 22
Plasmids
 pMM1pET19b/N-His-FlhBC 18
 pMM7pET19b/N-His-FLAG-FlhBThis study
 pMM12pET19b/N-His-FLAG-FlhBTM This study
 pMM14pET22b/C-His-FlhBC This study
 pMM15pET22b/untagged FlhBC This study
 pMM20pET19b/N-His-FlhBCC This study
 pMM23pTrc99A/untagged FlhBΔCC This study
 pMM24pET19b/N-His-FLAG-FlhBΔCC This study
 pMM25pTrc99A/untagged FlhBΔCC + N-His-FlhBCC This study
 pMM26pTrc99A/untagged FlhBThis study
 pMM28pTrc99A/N-His-FlhBCC This study
 pMM29pTrc99A/untagged FlhBTM + N-His-FlhBCC This study
 pMMBc2701NHpET19b/N-His-FlhBC of MY2701This study
 pMMBc2714NHpET19b/N-His-FlhBC of MY2714This study
 pMMBc3018NHpET19b/N-His-FlhBC of MY3018This study
 pMMBc3201NHpET19b/N-His-FlhBC of MY3201This study
 pMMBc3501NHpET19b/N-His-FlhBC of MY3501This study
 pMMBc3519NHpET19b/N-His-FlhBC of MY3519This study