Table 2.

(p)ppGpp formation under various conditionsa

Condition (p)ppGpp formation
Control
1% Oxygen
5 mM Sodium azide+
Stationary phase+
20 mM H2O2
Carbon starvation+/−
Complete starvation+++
1-mg/ml Serine hydroxamate
1-μg/ml Isoniazid
10-μg/ml Ethambutol
  • a M. tuberculosis cells were labeled as described in Materials and Methods, and nucleotides were resolved by 2D-TLC. (p)ppGpp formation was assessed by autoradiographic analysis and compared with that of authentic unlabeled (p)ppGpp.