Table 3.

Growth of OmpF mutants without DegP and complementation by DegPS210Aa

OmpF-340 residueGrowth without DegP at indicated temp of cells with:Complementation by DegPS210A in cells with Chr-OmpF in the presence of IPTGb at:
Plasmid-OmpFChr-OmpF
No IPTGWith IPTGb
30°C37°C30°C37°C30°C37°C30°C37°C
F340 (wtc)++++++++
Y340++++++NDND
L340++++++NDND
P340(−)(−)+++++
H340(−)(−)+++++
T340(−)(−)+++++
S340(−)(−)+++++
R340(−)(−)+++
A340++++++
  • a Plus sign, growth on all parts of the streak; minus sign, either no growth at all or no growth past the first streak on Luria agar medium; minus sign in parentheses, inability to construct OmpF mutants lacking DegP; Chr-OmpF, recombinant chromosomal alleles from which OmpF expression was controlled by an IPTG-inducible plasmid promoter; plasmid-OmpF, plasmid encoding OmpF; ND, not determined.

  • b IPTG concentration was 0.4 mM.

  • c wt, wild type.