Table 1.

Bacterial strains, plasmids, and oligonucleotides used in this study

Strain, plasmid, or oligonucleotideRelevant featuresReference
S. pneumoniae
 R6Subclone of R36A, derivative of D39 34
 KNR.7/87Serotype 4 clinical isolate; TIGR sequence 1
 D39Serotype 2; NCTC 7466 3
 1Clinical isolate; Roche strain collection 20
 1711Clinical isolate; Roche strain collection 20
 4241ATCC reference strainP. Geslin (Paris, France)
 LSP22R6hk13′::pAS1::′hk13 This study
Plasmids
 pJDC9Vector (Ery); nonreplicative in S. pneumoniae 7
 pAS1Ery; pJDC9 derivative 27
 pRPL54pAS1 derivative carrying a 401-bp fragment targeting hk13 (nt 456–857)This study
Oligonucleotides
 HK13EFGAGAAAGGGAATTCTCGATAAAGCThis study
 HK13RBCTGACTGGGATCCTATAAGACGGAGa This study
  • a Underlined nucleotides were modified to create an in-frame stop codon and an EcoRI restriction site.