Table 1.

Bacteria and plasmids used in this study

Strain or plasmidGenotype or descriptionReference or source
Strains
 MC4100araD139Δ(argF-lac)205 λflbB5301 ptsF25 relA1 rpsL150 deoC1CGSCa
 RYC1000MC4100 Δrib-7 recA56 gyrAF. Moreno
 POI1681TRF Mu dI1681 (Kmr)ara::(Mu cts)3Δ(proAB-argF-lacIPOZYA) XIII rpsL recA56 srl::Tn10 (Tcr)8
 ZK1171W3110 ΔlacU169 tna-2 rpoS::Tn10R. Kolter
 RO71MC4100 φ (csi-5::lacZ) (λplacMu55) ΔhimA82-Tn10R. Hengge-Aronis
 RO64MC4100lrp-201::Tn10R. Hengge-Aronis
 RH74MC4100 Δcya851 ilv::Tn10R. Hengge-Aronis
 ZK213Hfr rpsLR. Kolter
 ZK216ZK213 Δcrp45R. Kolter
 RO98MC4100 ΔrelA251::kanΔspoT207::catR. Hengge-Aronis
 MJ150MC4100 ΔhimA82-Tn10This study
 MJ152MC4100 ΔspoT207::catThis study
 MJ153MC4100rpoS::Tn10This study
 RYC514MC4100 ompR101F. Moreno
 CU284MC4100 hns::neoC. Ueguchi
 CC118FΔ(ara-leu)7697 araD139ΔlacX74 galE galK ΔphoA20 thi rpsE rpoB argE(Am) recA1 appR128
 CC170As CC118, with a chromosomal insertion of TnlacZ28
 pop3001.6MC4100 Mu ctsF. Moreno
Plasmids
 pTUC202pACYC184 with 6-kbBamHI-SalI fragment encompassingmcjABCD genes45
 pTUC341pBR322 with 5.2-kbHindIII-SalI fragment encompassingmcjABCD genes45
 pMcjA-LacZpTUC202 carryingmcjA::lacZ gene fusionThis study
 pMcjB-LacZpTUC202 carryingmcjB::lacZ gene fusionThis study
 pMcjC-LacZpTUC202 carryingmcjC::lacZ gene fusionThis study
 pMcjA-LacZ(op)pTUC202 carryingmcjA::lacZ operon fusionThis study
  • a CGSC, E. coli Genetic Stock Center.