Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidPhenotype or descriptionSource or reference
M. catarrhalis
 O35EWild-type strain19
 O35E.1Isogenic uspA1 mutant of O35E; expresses no UspA12
 O35E.98O35E isolate with poly(G) tract containing 10 G residues; expresses wild-type levels of UspA1This study
 O35E.118O35E isolate with poly(G) tract containing 10 G residues; expresses wild-type levels of UspA1This study
 O35E.117O35E isolate with poly(G) tract containing 9 G residues; expresses very low levels of UspA1This study
 O35E.121O35E isolate with poly(G) tract containing 9 G residues; expresses very low levels of UspA1This study
 O35E.135O35E isolate with poly(G) tract containing 9 G residues; expresses very low levels of UspA1This study
 O35E.137O35E isolate with poly(G) tract containing 9 G residues; expresses very low levels of UspA1This study
 O35E.118ATT1Derived from O35E.118 by selection for attachment to Chang cell monolayers; poly(G) tract has 10 G residuesThis study
 O35E.118ATT2Derived from O35E.118 by selection for attachment to Chang cell monolayers; poly(G) tract has 10 G residuesThis study
 O35E.118ATT3Derived from O35E.118 by selection for attachment to Chang cell monolayers; poly(G) tract has 10 G residuesThis study
 O35E.135ATT1Derived from O35E.135 by selection for attachment to Chang cell monolayers; poly(G) tract has 9 G residuesThis study
 O35E.135ATT2Derived from O35E.135 by selection for attachment to Chang cell monolayers; poly(G) tract has 9 G residuesThis study
 O35E.135ATT3Derived from O35E.135 by selection for attachment to Chang cell monolayers; poly(G) tract has 9 G residuesThis study
 O35E.118CATuspA1::catreporter strain; poly(G) tract contains 10 G residuesThis study
 O35E.135CATuspA1::catreporter strain; poly(G) tract contains 9 G residuesThis study
 O12EWild-type strain12
 O12E.44O12E isolate with poly(G) tract containing 10 G residues; expresses wild-type levels of UspA1This study
 O12E.77O12E isolate with poly(G) tract containing 9 G residues; expresses very low levels of UspA1This study
 O12E.1Isogenic uspA1 mutant of O12E; expresses no UspA126
 TTA37Wild-type isolate26
 O46EWild-type isolate26
E. coliDH5αHost strain for cloning experimentsNew England Biolabs
H. influenzae DB117Host strain for cloning experiments41
Plasmids
 pACYC184Cloning vectorNew England Biolabs
 pELU1-10 GpACYC184 containing the M. catarrhalis O35E.118 uspA1 gene with 10 G residues in the poly(G) tractThis study
 pELU1-8 GpACYC184 containing the M. catarrhalis O35E.118 uspA1 gene with 8 G residues in the poly(G) tract; obtained by site-directed mutagenesis of pELU1-10 GThis study
 pELU1-11 GpACYC184 containing the M. catarrhalis O35E.118 uspA1 gene with 11 G residues in the poly(G) tract; obtained by site-directed mutagenesis of pELU1-10 GThis study
 pELU1-3G4R3GpACYC184 containing the M. catarrhalis O35E.118 uspA1 gene with the poly(G) tract replaced by GGGACTAGGG; obtained by site-directed mutagenesis of pELU1-10 GThis study
 pELU1-3G4R2GpACYC184 containing the M. catarrhalisO35E.118 uspA1 gene with the poly(G) tract replaced by GGGACTAGG; obtained by site-directed mutagenesis of pELU1-10 GThis study
 pELU1-9 GpACYC184 containing the M. catarrhalis O35E.135 uspA1 gene with 9 G residues in the poly(G) tractThis study
 pELU1-NOGpACYC184 containing the M. catarrhalis O35E.135 uspA1 gene lacking the poly(G) tract and upstream DNAThis study
 pUSPA1pBluescript containing an incomplete uspA1gene from M. catarrhalis O35E2
 pELU1CATpUSPA1 with a promoterless cat cartridge inserted into the incomplete uspA1 geneThis study
 pSL1Source of the promoterless cat cartridge29