Table 2.

Adherence of M. catarrhalis strains and recombinant H. influenzae cells to Chang monolayers and detection of UspA1 on the bacterial cell surface

StrainLength of poly(G) tract (nt)UspA1 expressioncMean % adherence ± SD to Chang monolayersdMean detection (cpm) ± SD of UspA1 on cell surfacee
O35E.11810++++72.4 ± 17.4113,000 ± 14,300
O35E.1359+15.6 ± 10.313,780 ± 4,065
O35E.1NDa1.6 ± 0.21,731 ± 315
O35E.135CAT91.5 ± 0.5ND
O12E.4410++++126.4 ± 9.6103,032 ± 7,844
O12E.779+31.7 ± 14.617,078 ± 1,732
O12E.1ND4.3 ± 0.71,604 ±183
DB117(pELU1-10G)10+++26.6 ± 8.67,558 ± 304
DB117(pELU1-9G)9+1.4 ± 0.32,149 ± 885
DB117(pACYC184)NAb0.1 ± 0.1898 ± 198
  • a ND, not determined.

  • b NA, not applicable.

  • c The level of UspA1 expression was evaluated by colony blot RIA and Western blot analysis.

  • d Adherence to Chang conjunctival epithelial cells is expressed as the mean percentage of the inoculum which adhered to the monolayers. A total of six independent experiments were performed to obtain these data.

  • e Expressed as the mean counts per minute of radioiodinated goat anti-mouse immunoglobulin bound to MAb 24B5 (forM. catarrhalis isolates) or MAb 17C7 (for recombinantH. influenzae clones) on the bacterial cell surface.