Table 1.

Bacterial strains and plasmids

Strain or plasmidGenotypeSource or reference
E. coli strains
 HW272ubiG+zei::Tn10dTet42
 GD1ubiG::Kanzei::Tn10dTet18
 RM1734(MG1655)1 Frph-1 (wild type)30
 DM123RM1734yigR::Kan30
 AN59Hfr, thr-1 leu-6 ubiE409::IS112a
 LS1312fre::KanDiRussob;11
P. stuartii strains
 PR50Wild type30
 PR54PR50aarF::Cm30
Plasmids
 pET21aT7 expression plasmid, ApNovagen
 pEF1pET21a::3.5-kb Sau3A1 fragment containing E. coli ubiE and yigP andubiB genes30
 pSK-2.6pBluescript SK(−)::2.6-kb SalI fragment from pEF1 (yigP and ubiBgenes)30
 pSK-aarFpBluescript SK(−)::1.9-kb fragment from pAFM12 (P. stuartii aarF)30
 pQMVector containing the yeast CYC1 promoter18
 pUE-3pQM::ubiE coding region28
  • a The mutant allele in this strain has been characterized as ubiE409::IS1 and replaces the original designation “ubiB409.

  • b Department of Biochemistry and Molecular Biology, Albany Medical College, Albany, N.Y.