Table 1.

MICsa of copper for different genetic constructs of E. coli W3110

StrainGenotypeMIC of CuCl2 (mM)Source
W3110Wild type3.5Lab stock
GR1W3110 ΔcueO::cm 2.75b This work
GR5W3110 ΔcusA::cm 3.5This work
GR6W3110 ΔcusCFBA::cm 3.5This work
GR7W3110 ΔcueO::cm/pTYB2::cueO3.0This work
DW3110W3110copA::km 2.5b Rensing et al. (16)
GR8W3110copA::km ΔcueO::cm 2.25b This work
GR10W3110 ΔcueO ΔcusCFBA::cm 1.3c This work
GR12W3110 copA::km ΔcusCFBA::cm 2.5bThis work
GR13W3110 copA::km ΔcusA::cm 2.5b This work
GR15W3110 ΔcueO ΔcusA::cm 1.3c This work
GR16W3110 copA::km ΔcueO cusA::cm 1.3c This work
GR17W3110 copA::km ΔcueO cusCFBA::cm 1.3c This work
GR18W3110 ΔcueO ΔcusCFBA::cm/pTYB2::copA (R. metallidurans CH34)3.0This work
GR19W3110 ΔcueO ΔcusCFBA::cm/pACYC::cueO 3.0This work
GR20W3110 ΔcueO::cm/pACYC::cueO 3.5This work
GR21W3110 ΔcueO::cm/pTYB2::copA(R. metallidurans CH34)3.5This work
  • a The MIC is defined as the concentration at which no growth was observed. Overnight cultures were diluted 1:500 into fresh Luria-Bertani medium, and after 2 h of growth at 37°C, they were streaked out on Luria-Bertani plates with different concentrations of CuCl2. Growth was monitored after 16 h at 37°C.

  • b Colonies were small, and colorless, and sometimes mucoid at concentrations well below the MIC.

  • c Colonies were small and mucoid at 0.5 mM CuCl2.