Table 2.

Enantioselective oxidation of chiral alcohols by homogeneous THFA-DH from R. eutropha strain Boa

SubstrateKmb(mM)Vmax(U/mg)Kcat/Km × 104 (M−1s−1)Ki (mM)E
(±)-2-Butanol1.1228.23.1
R-(−)-2-Butanol1.1417.51.9
S-(+)-2-Butanol0.5932.06.63.5
(±)-2-Heptanol0.05896.8204.21.03
R-(−)-2-Heptanol1.1622.12.30.82
S-(+)-2-Heptanol0.046131.6350.00.93150.2
(±)-2-Octanol0.07680.4129.41.34
R-(−)-2-Octanol0.26918.28.36.62
S-(+)-2-Octanol0.051101.3243.00.9829.4
(±)-SolketalND
R-(−)-Solketal0.113.13.4
S-(+)-Solketal0.745.60.90.27
  • a The kinetic parameters were determined using ferricyanide as an artificial electron acceptor and under the conditions described in Table 1, footnote a. The enantiomeric ratios are expressed in E values as described in Materials and Methods. —, no substrate inhibition detected under the conditions used.

  • b The curves were fitted to the model of Michaelis and Menten with or without substrate inhibition. ND, not determined.