Table 1.

EPR properties of wild-type Isf and variants

ProteinApparentg valuesIron-sulfur center type
Wild type
 As purified2.06, 1.92, 1.81 (species I)[4Fe-4S]
2.03, 1.92, 1.86 (species II)
 Reconstituted2.06, 1.92, 1.81 (species I)[4Fe-4S]
2.03, 1.92, 1.86 (species II)
Variants
 C16A/S2.06, 1.92, 1.82 (species I)[4Fe-4S]
2.04, 1.92, 1.86 (species II)
 C47A2.05, 1.93, 1.89[4Fe-4S]
 C50A2.01, 1.98[3Fe-4S]
 C53A2.03, 1.91, 1.89[4Fe-4S]
 C59A2.02, 1.99[3Fe-4S]
 C180A/S2.04, 1.93, 1.87; with minor contribution from 2.06, 1.93, 1.81[4Fe-4S]