Table 1.

Bacterial strains used in this study

Species and strainGenotypeSource or referencea
B. subtilis
 168 trpC2 Wild-type strain
 BFS45 trpC2Pytr::pMUTIN2mcsThis work (pytrA3B→168)
 BFS47 trpC2 ytrF-pMUTIN2mcsThis work (pytrF1→168)
 BFS53 trpC2 ΔytrA::pMUTIN2mcsThis work
 FU349 trpC2 ytrF::pMUTIN2mcsThis work (pytrFd→168)
E. coli C600 supE44 hsdR thi-1 thr-1 leuB6 lacY1 tonA21 30
  • a Arrows indicate transformation from donor DNA to recipient strain.