Table 1.

Bacterial strains and plasmids

Strain or plasmidGenotype or relevant characteristicsaSource or reference
S. aureus
 ISP7948325pig-13124
 MT231428325pig-131 flqB16
 RN42208325-4 r12
E. coli
 DH5αFφ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 phoA hsdR17(rK mK)supE44 λthi-1 gyrA96 relA1Gibco-BRL
Plasmids
 pWN201810.5-kbS. aureus transcriptional promoter-probe vector, Cmr27
 pBF8-30Transcriptional fusion of wild-type promoter (315 bp) with blaZ gene of pWN20185
 pBF5-10Transcriptional fusion of wild-type promoter (242 bp) with blaZ gene of pWN2018This study
 pBF15-5Transcriptional fusion of wild-type promoter (110 bp) with blaZ gene of pWN20185
 pBF7-7Transcriptional fusion of flqB promoter (315 bp) with blaZ gene of pWN2018This study
 pWN181810.5-kb S. aureus translational promoter-probe vector, Cmr27
 pBF2Translational fusion of wild-type promoter (342 bp) withblaZ gene of pWN1818This study
 pBF1Translational fusion of flqB promoter (342 bp) with blaZ gene of pWN1818This study
  • a Cm, chloramphenicol.