Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotypeSource or reference
P. gingivalis
 A7436Wild typeLab collection
 381Wild typeLab collection
 W50Wild typeT. van Dyke, Boston University, Boston, Mass.
 ATCC 33277Wild typeLab collection
 ATCC 53977Wild typeP. Baker, Bates College, Lewiston, Maine
 WS1A7436,hmuR::erm This study
E. coli
 DH5α recA1 lacZYA-argF supE44 Promega
 TOP10F′F′ [lacl q Tn10(Tetr)]mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 deoR araD139(ara-leu)7697 galU galK rpsL (Strr)endA1 nupG Invitrogen
 BL21(DE3)pLysEF ompT hsdS B(rB mB ) gal dcm (DE3)pLysE (Camr)Invitrogen
Plasmids
 pGEM3z(−)Ampr Promega
 pWS1pGEM3z(−) containing 485-bp N terminus ofhmuR and the B. fragilis ermF cassette within thePstI site of the hmuR geneThis study
 pCRT7/CT-TOPOAmpr Invitrogen
 pTO1pCRT7/CT-TOPO containing the hmuR gene without the signal peptide sequenceThis study
 pTO2pCRT7/CT-TOPO containing the hmuR gene with the signal peptide sequenceThis study