Table 2.

Double-disruption selection experiments

LocusStrain (genotypea)Detection primersUra+ rate per divisionbArg+ Ura+rate per divisionbNo. of homozygotesc
ADE2BMY7 (ade2::UAU1/ADE2)Ade2amp5, Ade2amp3, Arg4det3.5 × 10−62.0 × 10−812
YGR189DAY151 (ygr189::UAU1/YGR189)Ygr189amp5, Ygr189amp3, Arg4det2.3 × 10−61.0 × 10−810
RIM20BMY17 (rim20::UAU1/RIM20)Rim20amp5, Rim20amp3, Arg4det1.5 × 10−56.8 × 10−811
SNF1BMY18 (snf1::UAU1/SNF1)Snflamp5, Snflamp3, Arg4det2.9 × 10−61.3 × 10−80
CDC28BMY22 (cdc28::UAU1/CDC28)Cdc28amp5, Cdc28amp3, Arg4det7.9 × 10−63.5 × 10−70
CDC25BMY16 (cdc25::UAU1/CDC25)Cdc25amp5, Cdc25amp3, Arg4det5.4 × 10−63.6 × 10−92
  • a Strains carried the additional mutations ura3Δ::λimm434/ura3Δ::λimm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG.

  • b Recombination rates were calculated through the method of the median, as described in Materials and Methods.

  • c The number of homozygous mutants found among 30 independent Arg+ Ura+ segregants from the heterozygote indicated. Genotypes were determined through PCR analysis, examples of which are shown in Fig. 2.