Table 1.

Properties of the gene products involved in anaerobic phenol metabolism and similarities with other proteins in the databasesa

Gene productSimilar proteinbGene propertiescProtein propertiesdProtein correspondenceeProtein with highest similarityf
DirRangeLength (aa)Mol mass (kDa)pI% Identity% SimilarityE valueReferencesProtein
DeducedExpDeducedExp
Orf11(7)688–243658263.756556.146.547.272.31E−20gnl‖PID‖d1010531 (D63814) DmpR (transcriptional activator)
Orf1F32864–470261270.261675.065.516.739.34E−10gi‖147146 (M69116) PEP synthase (central part)
Orf2(6)4716–584037440.423405.385.521.834.51E−63gi‖147146 (M69116) PEP synthase (N-terminal part)
Orf3(9)5853–652422324.244306.196.514.530.21E−8gi‖2621183 (AE000803) Inosine-5′-monophosphate dehydrogenase
Orf4F26587–800547252.605586.067.030.858.955E−47gi‖549586‖sp‖P26615‖UbiD3-Octaprenyl-4-hydroxybenzoate carboxy lyase
Orf5F48070–857916918.450185.626.538.863.82E−25gi‖2851406‖sp‖P45396‖YrbIHypothetical protein
Orf6F18616–1007348553.940605.646.529.457.11E−31gi‖549586‖sp‖P26615‖UbiD3-Octaprenyl-4-hydroxybenzoate carboxy lyase
Orf7(8)10731–1180435738.198356.687.024.747.57E−25gi‖549586‖sp‖P26615‖UbiD3-Octaprenyl-4-hydroxybenzoate carboxy lyase
Orf8F511819–1240319421.262206.978.060.386.85E−56gi‖2507150‖sp‖P09550‖UbiX3-Octaprenyl-4-hydroxybenzoate carboxy lyase
Orf9(11)12414–1284514315.209177.609.040.064.88E−13gi‖2622617 (AE000910) Hypothetical protein
Orf10(10)12884–1343218219.936185.765.536.162.72E−9gi‖2129134‖pir‖D64443Mutator protein MutT
  • a The deduced properties of the gene products of orf1 to orf11 (as determined by diverse computer programs) are compared with the properties of phenol-induced proteins detected in two-dimensional gels.

  • b Numbers in parentheses refer to the numbers of the spots of corresponding proteins in a two-dimensional gel in Fig.2.

  • c Dir, direction of transcription. The range is of the nucleotides of the corresponding gene.

  • d Mol mass, molecular mass; pI, isoelectric point; Exp., experimentally determined (two-dimensional gels); aa, amino acids. The length is the number of amino acids of the corresponding translated gene product.

  • e Overall similarity and identity of the amino acid sequence to proteins in the databases are given. % Identity, the percentage of amino acids that are identical between the two proteins; % Similarity, the percentage of amino acids that are identical or conserved between the two proteins; E value, Expect value. The Expect value estimates the statistical significance of the match, specifying the number of matches, with a given score, that are expected in a search of a database of this size absolutely by chance.

  • f Given are references of similar proteins. Database accession numbers are in parentheses.