Table 1.

Strains, phages, and plasmids

Strain, phage, or plasmidDescriptionSource or reference
Strains
 DH5αWild-type, F endA1 hsdR17 supE44 thi-1 recA1 deoR gyrA96 relA1 Δ(argF-lacZYA)U169φ80dlacZΔM15 λ New England Biolabs
 DLACus, mutant of parent strain DH5αThis study
 DLBCus, mutant of parent strain DH5αThis study
 DLGCus, ΔcusRS, mutant of parent strain DH5αThis study
 DLHCus, mutant of parent strain DH5αThis study
 DLICus, mutant of parent strain DH5αThis study
 DLJCus, ΔcusRS, mutant of parent strain DH5αThis study
 DLKCus, ΔcusRS, mutant of parent strain DH5αThis study
 DLNCus, mutant of parent strain DH5αThis study
Phages
 λPpcoE-lacZ Reporter phage carrying PpcoE −70 to +31acloned upstream of promoterless lacZ of λRS45This study
 λPpcoA-lacZ Reporter phage carrying PpcoA −576 to +405a cloned upstream of promoterless lacZ of λRS45This study
 λPcusC-lacZ Reporter phage carrying PcusC −114 to +12a cloned upstream of promoterless lacZ of λRS45This study
λRS45Reporter phage carrying promoterlesslacZ 33a
Plasmids
 pCOVI133 pcoRS cloned into pSX34lacZαThis study
 pCOIV199-D7 pcoE-lacZgene fusion cloned into pRS414 expressed from PpcoE−348 to +186a This study
 pPA87 pcoABCDRSE cloned into pBR32232
 pCL27-1 cus, 2-kb Sau3Al fragment of E. coli chromosome cloned into BamHI site of pSX34NoHindIIIThis study
 pCL115-1 cus, 6-kbNsiI-BamHI fragment of E. colichromosome cloned into PstI-BamHI sites of pSX34LacZαThis study
 pCOIV239-B1 pcoABCDRSE, 7.5-kb HindIII-BglII fragment from pPA87 cloned into HindIII-BamHI sites of pSX34LacZαThis study
 pRS414Promoterless lacZ cloning vector33a
 pSX34LacZαLow-copy-number cloning vectorNew England Biolabs
 pSX34NoHindIIILow-copy-number cloning vector, derivative of pSX34LacZα withoutlacZα complementationThis study
  • a Numbering is relative to the transcription start site.