Table 2.

Expression of the acoABCL operon andacoR

StrainRelevant genotypeβ-Galactosidase specific activity (Miller units/mg of protein)
CSK CSK + 10 mM acetoin CSK + 10 mM acetoin + 0.5% glucose
QB7700 acoA′-′lacZ 14548,975 655
QB7701 acoA′-′lacZ ccpA::spc 535 55,210 50,035
QB7704 acoA′-′lacZ acoR::aphA3 10 10 3
QB7702 acoA′-′lacZ sigL::aphA3 5 2 3
QB7713 acoR′-′lacZ 155 140 10
QB7714 acoR′-′lacZpACOR1 125 145 5
QB7724 acoA′-′lacZ pMutin4 355 3,875 NDa
QB7725 acoB′-′lacZ pMutin4 470 4,230 ND
QB7726 acoC′-′lacZ pMutin4 954,740 ND
QB7727 acoL′-′lacZpMutin4 45 3,765 ND
QB7733 acoR′-′lacZ pACOR1ccpA::spc 215 230220
  • a ND, not determined.