Table 4.

SOS responses induced by transposase and mini-IS1

PlasmidaHostbPresence of arabinosecLacZd(U)Net increasee
pYS10Twt+6833
35
hns +39−1
40
pYS30wt+3911
28
hns +351
34
Nonewt+58−2
60
hns +52−2
54
wt+f 6837
31
hns +f 10673
33
  • a For the structures of pYS10T and pYS30, see Fig. 2.

  • b Host strains were CSH26 (wt) and YS28 (hns), both of which carrysulA::lacZ. wt, wild type.

  • c Cells were grown in the presence (+) or absence (−) of arabinose.

  • d β-Galactosidase (LacZ) activity in the cell lysate. Each value is the mean of those obtained from three independent experiments; standard errors in all cases were less than 4.7%.

  • e Net increase in β-galactosidase activity.

  • f Mitomycin C (final concentration, 5 ng/ml) was added instead of arabinose.