Table 1.

List of bacterial strains and plasmids used in this study

Strain or plasmidGenotype and relevant markerSource
Strains
B. abortus
  S2308 B. abortus wild-type bovine isolate, Nalr B. L. Deyoe
  BA582S2308 cydB::Tn5Nalr Kmr This study
E. coli
  DH10BF mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15ΔlacX74 deoR recA1 araΔ139Δ(ara leu)7697 galU galKI rpsL endA1 nupG Gibco BRL
Plasmids
 pBBR1MCS.cosCloning vector, modified from pBBR1MCSC. A. Allen
 pSEK101 B. abortus cydAB XbaI fragment in pBBR1MCS.cosThis study
 pSEK102 B. abortus cydAB HindIII fragment in pBBR1MCS.cosThis study
 pSEK103 B. abortus ΔcydA BamHI fragment in pBBR1MCS.cosThis study
 pMEK15 B. abortus Cu/Zn SOD gene on pBBR1MCSM. Roop
 pMEK21 B. abortuscatalase gene on pBBR1MCSM. Roop
 pBluescript KS IIColE1bla Stratagene