Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidReference or sourceComments
S. epidermidis
 145731Isolate from infected central venous catheter
 M1230Isogenic biofilm-negative Tn917 mutant of S. epidermidis1457 (class II)
 M1530Isogenic biofilm-negative Tn917 mutant of S. epidermidis 1457 (class III)
 M1930Isogenic biofilm-negative Tn917 mutant ofS. epidermidis 1457 (class III)
 M16This studyIsogenic biofilm-positive control Tn917 mutant ofS. epidermidis 1457
 840031Blood culture isolate
 8400-M15This studyTransductant of S. epidermidis 8400
 517931Blood culture isolate
 RP62A7
 SE542
 52127Isolate from infected central venous catheter
 105727Isolate from infected central venous catheter
 93927Blood culture isolate
 1033331Blood culture isolate
 783731Blood culture isolate
 922531Blood culture isolate
 989631Blood culture isolate
S. aureus 35005This studyClinical isolate
E. coli
 MC106112
 TOP10Invitrogen
 JKMK186-41This studyMC1061 containing pJKMK186-41
 JKMK186-46This studyMC1061 containing pJKMK186-46
 JKMK401This studyTOP10 containing pJKMK401
 JKMK402This studyTOP10 containing pJKMK402
Plasmids
 pBluescript II SKInvitrogen E. coli cloning vector for blue-white screening
 pCRIIInvitrogen E. coli cloning vector for direct cloning of PCR fragments
 pJKMK186-41This studypBluescript II SK containingHindIII/SalI fragment of 1457-M15 with chromosomal DNA flanking 5′ end (erm) of Tn917
 pJKMK186-46This studypBluescript II SK containingHindIII/SalI fragment of 1457-M19 with chromosomal DNA flanking 5′ end (erm) of Tn917
 pJKMK401This studypCRII containing sigBamplification product obtained from S. epidermidis 1457 with oligonucleotides JKMK4 and JKMK5
 pJKMK402This studypCRII containing amplification product obtained with oligonucleotides 3R and 5L (sigB operon of S. epidermidis 1457)