Table 1.

Bacterial strains and plasmids

Strain, plasmid, or phageRelevant characteristicsaSource or reference
P. aeruginosa
 PT5PAO1, wild typeLaboratory collection
 PT454PT5ΔrhlI::Tn501, transduced from PDO100 6, this study
 PT462PT5rhlR::Tn501, transduced from PAO-JP3 6, this study
 PT466PT5ΔlasI::Tc, transduced from PAO-JP1 42, this study
 PT498PT5ΔlasR::Tc, transduced from PAO-JP3 42, this study
 PT610PAKS. Lory
 PT611PAK-R1, pilR::GmS. Lory
 PT612PT5pilR::Gm, transduced from PAK-R1 24, this study
 PT613PAK-NP,pilA::Tc 45
 PT623PT5pilA::Tc, transduced from PAK-NP 45, this study
 PT688PAO1fliC::GmR. Ramphal
 PT690PT5fliC::GmThis study
 PT711PAO1ΔrhlA::GmU. Ochsner
 PT712PT5ΔrhlA::Gm, transduced from PT711This study
Plasmids
 pMMB207Broad-host-range vector, Cmr 32
 pKI23 ptac::pilR in pMMB67EH, Apr 4
 p207R1 ptac::pilR in pMMB207, Cmr This study
 pJPP6 rhlABRI in pBSIISK+, Apr J. P. Pearson
 pJMC30 lasRI-carrying pUC derivative, Apr 39
Bacteriophage E79tv2 LPS-specific transducing phage 33
  • a Tc, tetracycline; Cm, chloramphenicol; Ap, ampicillin; Gm, gentamicin; LPS, lipopolysaccharide.