Table 1.

Bacterial strains and plasmids

Strain, plasmid, or phageRelevant characteristicsaSource or reference
P. aeruginosa
 PT5PAO1, wild typeLaboratory collection
 PT454PT5ΔrhlI::Tn501, transduced from PDO1006, this study
 PT462PT5rhlR::Tn501, transduced from PAO-JP36, this study
 PT466PT5ΔlasI::Tc, transduced from PAO-JP142, this study
 PT498PT5ΔlasR::Tc, transduced from PAO-JP342, this study
 PT610PAKS. Lory
 PT611PAK-R1, pilR::GmS. Lory
 PT612PT5pilR::Gm, transduced from PAK-R124, this study
 PT613PAK-NP,pilA::Tc45
 PT623PT5pilA::Tc, transduced from PAK-NP45, this study
 PT688PAO1fliC::GmR. Ramphal
 PT690PT5fliC::GmThis study
 PT711PAO1ΔrhlA::GmU. Ochsner
 PT712PT5ΔrhlA::Gm, transduced from PT711This study
Plasmids
 pMMB207Broad-host-range vector, Cmr32
 pKI23ptac::pilR in pMMB67EH, Apr4
 p207R1ptac::pilR in pMMB207, CmrThis study
 pJPP6rhlABRI in pBSIISK+, AprJ. P. Pearson
 pJMC30lasRI-carrying pUC derivative, Apr39
Bacteriophage E79tv2LPS-specific transducing phage33
  • a Tc, tetracycline; Cm, chloramphenicol; Ap, ampicillin; Gm, gentamicin; LPS, lipopolysaccharide.