Table 1.

Distribution of the inserted sequence among clinicalE. coli strains

Strain or isolate(s)OriginPCR test 1aPCR test 2bSource or reference
putP(202 bp)Internal fragment (301 bp)rpoS border (579 bp)o454 border (483 bp)
ATCC25992Clinical++++G. Reid
BB593:2bFecal (child)++++G. Reid
Co1Fecal (non-UTI)+G. Reid (35)
SM47c Neonatal meningitis+G. Reid
2 Isolatesd Hemorrhagic colitis+C. L. Gyles
3 IsolatesHemorrhagic colitis+NTNTC. L. Gyles
3 Isolatesd Infantile diarrhea+C. L. Gyles
16 IsolatesInfantile diarrhea+NTNTC. L. Gyles
2 IsolatesInfantile diarrhea++++C. L. Gyles
1 Isolatec Catheter+G. Reid
3 Isolatesd Catheter+G. Reid
3 IsolatesCatheter++++G. Reid
431Bacteriuria++++G. Reid (35)
950UTI (bacteremia)++++G. Reid (38)
2239UTI++++G. Reid
C1212UTI++++G. Reid (31)
C1214UTI++++G. Reid (31)
2 IsolatesCystitis+G. Reid
2 Isolatesd Cystitis+G. Reid (33, 34)
8 IsolatesCystitis++++G. Reid (33, 34)
CFT073Acute pyelonephritis++++H. Mobley (27)
HU734Acute pyelonephritis++++G. Reid (16, 17)
5 IsolatesPyelonephritis++++G. Reid
  • a Multiplex PCR test 1 was performed withputP primers plus primers C and D (Fig. 2). TheputP primers were putP1, 5′-GGTTGCGTGTGCATACCGA-3′ (bp 287 to 305 of putP), and putP2, 5′-GCCGTTTCGTAGCTCATGC-3′ (bp 469 to 488 ofputP). +, DNA fragments of the indicated sizes were obtained; −, PCR yielded no DNA fragment.

  • b Multiplex PCR test 2 was performed using primers A, B, E, and F (Fig. 2). +, DNA fragments of the indicated sizes were obtained; −, PCR yielded no DNA fragment; NT, PCR test 2 was not performed.

  • c Multiplex PCR test 2 yielded a single 526-bp DNA fragment (characteristic of E. coli K-12).

  • d Multiplex PCR test 2 yielded a DNA fragment pattern unlike that of E. coli CFT073 or E. coliMC4100.