Table 2.

Purification of Xfp from B. lactis

StepVol (ml)Total unitsTotal protein (mg)Sp act (Ua/mg)Purification factorYield (%)
Crude extract1593.4246.00.381.0100
DEAE-cellulose13828.747.10.611.630.7
Mono Q2.115.57.42.105.516.6
Superdex 2006.04.10.964.2811.14.4
  • a One unit is defined as the amount of extract forming 1 μmol of acetyl phosphate per min with F6P as a substrate.