Table 3.

TnphoA and Tn10dCm swarming mutantsa

Type of function affected and mutantbSwarming phenotype on:P22 resistancecMutant genedFunctionReference
Difco mediumEiken medium
 8-11* +s hisD Histidine biosynthesis 19
 17-7* +s hisD Histidine biosynthesis 19
 64-7* +s hisD Histidine biosynthesis 19
 16-48* +s cybC Cytochrome synthesis 96
 L6-393+s dhnA Carbohydrate metabolism 94
 S28-14+s purH Purine biosynthesis 20
 S122-27+s iadA Peptide metabolism 30
 M151-44+s fadH Fatty acid metabolism 106
 M176-16+s fdhF Formate metabolism 104
Surface or membrane
 16-6* +s wcaK Capsular polysaccharide region 87
 AF20* +r waaKe LPS biosynthesis 73
 58-19* +r waaPe LPS biosynthesis 73
 L9-50+s shdA Similar to adhesinGenBank accession no. AF140550
 S82-3+s ompS1 Outer membrane protein 25
 S126-45+s sfiX Cell wall biosynthesis 27
 M17-48+s marA Membrane permeability 90
 M210-43+s corA Magnesium transport 43
 L5-192+s baeS Sensor kinase 68
 M208-35+s yfhK Putative sensor kinase 13
 S28-31+s yojN Putative sensor kinase 13
 L5-225+s xerC Chromosome segregation 12
 M112-47+s ydhC Unknown 21
 M153-43+s yihP Unknown 13
 M156-250+s ybeS Unknown 13
 M17-39+s yehE Unknown 13
  • a A total of 35,000 mutants (10,000 TnphoA and 25,000 Tn10dCm) were screened. There were 311 swarm-negative mutants (235 LPS, 44 Che, and 32 others), and 34 were sequenced.

  • b Asterisks indicate TnphoAinsertions; all others have Tn10dCm insertions.

  • c r, resistant; s, sensitive

  • d UN-1 and UN-2, contiguous sequences (contigs) identified in the S. enterica serovar Typhimurium (Washington University) and S. typhi (BLAST) unfinished genome databases, respectively.

  • e LPS core modification.