Table 3.

TnphoA and Tn10dCm swarming mutantsa

Type of function affected and mutantbSwarming phenotype on:P22 resistancecMutant genedFunctionReference
Difco mediumEiken medium
Metabolism
 8-11*+shisDHistidine biosynthesis19
 17-7*+shisDHistidine biosynthesis19
 64-7*+shisDHistidine biosynthesis19
 16-48*+scybCCytochrome synthesis96
 L6-393+sdhnACarbohydrate metabolism94
 S28-14+spurHPurine biosynthesis20
 S122-27+siadAPeptide metabolism30
 M151-44+sfadHFatty acid metabolism106
 M176-16+sfdhFFormate metabolism104
Surface or membrane
 16-6*+swcaKCapsular polysaccharide region87
 AF20*+rwaaKeLPS biosynthesis73
 58-19*+rwaaPeLPS biosynthesis73
 L9-50+sshdASimilar to adhesinGenBank accession no. AF140550
 S82-3+sompS1Outer membrane protein25
 S126-45+ssfiXCell wall biosynthesis27
 M17-48+smarAMembrane permeability90
 M210-43+scorAMagnesium transport43
Signaling
 L5-192+sbaeSSensor kinase68
 M208-35+syfhKPutative sensor kinase13
 S28-31+syojNPutative sensor kinase13
Other
 L5-225+sxerCChromosome segregation12
 M112-47+sydhCUnknown21
 M153-43+syihPUnknown13
 M156-250+sybeSUnknown13
 M17-39+syehEUnknown13
 L7-85+sUN-1
 S71-32+sUN-1
 S80-24+sUN-2
 S116-25+sUN-2
 S118-35+sUN-1
 M16-43+sUN-1
 M156-29+sUN-2
 M209-40+sUN-1
  • a A total of 35,000 mutants (10,000 TnphoA and 25,000 Tn10dCm) were screened. There were 311 swarm-negative mutants (235 LPS, 44 Che, and 32 others), and 34 were sequenced.

  • b Asterisks indicate TnphoAinsertions; all others have Tn10dCm insertions.

  • c r, resistant; s, sensitive

  • d UN-1 and UN-2, contiguous sequences (contigs) identified in the S. enterica serovar Typhimurium (Washington University) and S. typhi (BLAST) unfinished genome databases, respectively.

  • e LPS core modification.