Table 2.

TnphoA screen: Pho+ strains affected in swarminga

Type of function affected and mutantSwarming phenotype on:P22 resistancebMutant geneFunctioncReferenced
Difco mediumEiken medium
Surface or membrane
 11-50+r waaLe LPS biosynthesis
 41-11+r waaLe LPS biosynthesis
 42-6+r waaLe LPS biosynthesis
 44-35+r waaLe LPS biosynthesis
 47-34+r waaLe LPS biosynthesis
 46-17+r waaCf LPS biosynthesis
 2-47+r wbaPe LPS biosynthesis
 44-28+r wbaPe LPS biosynthesis
 50-22+r wbaPe LPS biosynthesis
 57-39+r wzxe LPS biosynthesis
 15-31+r ddhBe LPS biosynthesis
 24-9+r ddhBe LPS biosynthesis
 3-10+s mdoG MDO biosynthesis 54
 4-6+s mdoG MDO biosynthesis 54
 38-34+s mdoH MDO biosynthesis 54
Other
 2-7−/++s ptsH PTS pathway 101
 6-7Dpsg +s parB Virulence plasmid partitioning 79
 2-44+s yihG Unknown 13
  • a Five thousand blue colonies were screened. There were 35 swarm-negative mutants, and 18 were sequenced.

  • b r, resistant; s, sensitive.

  • c MDO, membrane-derived oligosaccharides; PTS, phosphotransferase system.

  • d For all unreferenced LPS genes, see reference73.

  • e LPS O-antigen synthesis.

  • f LPS core synthesis.

  • g Dps, defective in progressive swarming.