Table 2.

TnphoA screen: Pho+ strains affected in swarminga

Type of function affected and mutantSwarming phenotype on:P22 resistancebMutant geneFunctioncReferenced
Difco mediumEiken medium
Surface or membrane
 11-50+rwaaLeLPS biosynthesis
 41-11+rwaaLeLPS biosynthesis
 42-6+rwaaLeLPS biosynthesis
 44-35+rwaaLeLPS biosynthesis
 47-34+rwaaLeLPS biosynthesis
 46-17+rwaaCfLPS biosynthesis
 2-47+rwbaPeLPS biosynthesis
 44-28+rwbaPeLPS biosynthesis
 50-22+rwbaPeLPS biosynthesis
 57-39+rwzxeLPS biosynthesis
 15-31+rddhBeLPS biosynthesis
 24-9+rddhBeLPS biosynthesis
 3-10+smdoGMDO biosynthesis54
 4-6+smdoGMDO biosynthesis54
 38-34+smdoHMDO biosynthesis54
Other
 2-7−/++sptsHPTS pathway101
 6-7Dpsg+sparBVirulence plasmid partitioning79
 2-44+syihGUnknown13
  • a Five thousand blue colonies were screened. There were 35 swarm-negative mutants, and 18 were sequenced.

  • b r, resistant; s, sensitive.

  • c MDO, membrane-derived oligosaccharides; PTS, phosphotransferase system.

  • d For all unreferenced LPS genes, see reference73.

  • e LPS O-antigen synthesis.

  • f LPS core synthesis.

  • g Dps, defective in progressive swarming.