Table 2.

Expression of pel and out genes in the presence of an opgG mutation in E. chrysanthemia

EnzymeFusionInducerSp act (nmol of product liberated/min/mg (dry wt) ± SD
opgG+opgG
Pectate lyase0.05 ± 0.020.02 ± 0.01
+19.7 ± 3.28.4 ± 2.1
β-Galactosidase pelE-lacZ 7 ± 35 ± 2
+132 ± 1855 ± 9
pelC-lacZ 5 ± 22 ± 2
+863 ± 82432 ± 25
outC-lacZ 87 ± 558 ± 6
+340 ± 20156 ± 32
  • a Cultures were grown in minimal medium to late log phase in the presence of either glycerol (−) or the inducing compounds polygalacturonate and galacturonate (+). The results reported are averages of at least three independent experiments ± standard deviation. Pectate lyase specific activity reflects the expression of the five major pel genes (pelA topelE). β-Galactosidase specific activity reflects the expression of the corresponding lacZ fusion.