Table 1.

Bacterial strains and plasmids

Bacterial strain or plasmidDescriptionSource or reference
Bacterial strains
E. coli
  DH5α supE44 hsdR17 recA1 endA1 gyrA96 thi-1 ΔlacU169 relA1(φ80lacZΔM15)BRL
  JM109λpir recA1 endA1 gyrA96 thi hsdR17 supE44 relA1Δ(lac-proAB +) [F′ traD36,proAB lacI q lacZΔM15] λpir Lab collection
V. anguillarum
  775(pJM1)Wild type; Pacific Ocean prototype 19
  531A(pJHC1)Wild type; Atlantic Ocean prototype 49
  S531A-1Plasmidless derivative of 531AThis work
  531A(pJHC1δ)pJHC1 deletion derivative; TAF This work
  531A(pJHC1::Ω)pJHC1 derivative containing the Ω transcription-translation terminator at the BglII site in angB; B G This work
  531A(pJHC1::K)pJHC1 derivative containing a Klenow modification at the BglII site in angB; B G+ This work
  531A(pJHC1::ORFFδ)pJHC1 derivative containing an in-frame deletion in ORF FThis work
Plasmids
 pJM1Indigenous plasmid in strain 775 17
 pJHC1Indigenous plasmid in strain 531A 49
 pJHC-S100Kmr-encoding cloning vector derived from pBR325 37
 pBluescript SKCloning vectorStratagene
 pBR322Cloning vector 8
 pTW99pBR322 with kanamycin resistance gene from pUC4K cloned into SalI siteThis work
 pTW100TAFb region cloned as aBamHI-SalI fragment into pJHC-S100This work
 pTW104Derivative of pTW100 containing ORF B as anEcoRV-SpeI fragmentThis work
 pTW105pTW104 with the Ω fragment cloned into theBglII siteThis work
 pTW200 angB andangG cloned by PCR into pTW99This work
 pTW201 angB cloned by PCR into pTW99This work
 pTW202 EcoRV-BglII deletion derivative of pTW200This work
 pTW203pTW200 containing a Klenow modification at the BglII site in angB This work
 pTW200S248LpTW200 containing an S248L mutation introduced by site-directed mutagenesisThis work
 pTW203S248LpTW203 containing an S248L mutation introduced by site-directed mutagenesisThis work
 pQE60T5 phage promoter expression vectorQiagen
 pQE60-B+G+ angB and angG cloned into pQE60This work
 pQE60-BG+::KpQE60-B+ G+containing a Klenow modification at the BglII site inangB This work