Table 1.

Bacterial strains and plasmids used in this work

Strain or plasmidRelevant characteristicsSource or reference
Strains
A. vinelandii
  AEIVWild type, mucoidSvein Valla
  AEA8AEIV with a nonpolar mucA::Gm mutationThis work
  AEA4AEIV with a polar mucA::Gm mutationThis work
  AEC2AEIV with a nonpolarmucC::Tc mutationThis work
  ATCC 9046Highly mucoid due to the muc-1 spontaneous mutation 26
  JRA8ATCC 9046 with a nonpolar mucA::Gm mutationThis work
  JRA4ATCC 9046 with a polar mucA::Gm mutationThis work
  MLC2ATCC 9046 with a nonpolarmucC::Tc mutationThis work
  MLC4ATCC 9046 with a polar mucC::Tc mutationThis work
  WI12 algD::lacZ derivative of ATCC 9046 6
  WIA8WI12 with a nonpolar mucA::Gm mutationThis work
  WIA4WI12 with a polar mucA::Gm mutationThis work
  WIC2WI12 with a nonpolarmucC::Tc mutationThis work
  WIC4WI12 with a polar mucC::Tc mutationThis work
E. coli
  DH5α supE44 ΔlacU169 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 18
  CAG16037MC1061 λ(rpoH p3-lacZ) 29
  CAG22216CAG16037;rpoE::ΩCm 39
Plasmids
 pBluescript SK(+)Plasmid used for subcloning DNAStratagene
 pMOSBlue Plasmid used for subcloning PCR productsAmersham
 pHP45Ω-TcPlasmid used to obtain the Tcr cassette 15
 pBSL141Plasmid used to obtain the Gmrcassette 1
 pLRApMOSBluederivative carrying a 1.3-kb DNA fragment containing A. vinelandii mucA gene amplified by PCRThis work
 pLRA8pLRA derivative containing mucA::Gm nonpolar mutationThis work
 pLRA4pLRA containing mutationmucA::Gm polar to mucBCD This work
 pLRCpMOSBlue derivative carrying a 1.3-kb DNA fragment containing A. vinelandii mucC gene amplified by PCRThis work
 pLRC2pLRC derivative containingmucC::Tc nonpolar mutationThis work
 pLRC4pLRC derivative containing mutationmucC::Tc polar to mucD This work
 pJMSAT1Plasmid containing A. vinelandii algU-mucAgenes; Ap 34