Table 2.

Alginate production in different A. vinelandii strains

StrainGenotypeMean alginate concna (mg/mg of protein) ± SEM (%)Mucoidyb
AEIVWild type0.9 ± 0.2 (100)+
AEA8AEIV, mucA 5.9 ± 0.4 (651)+++
AEA4AEIV, mucABCD 4.0 ± 0.15 (444)++++
AEC2AEIV, mucC 2.4 ± 0.4 (266)++
ATCC 9046Wild type, muc-1 4.8 ± 0.3 (100)+++
JRA8ATCC 9046, mucA 6.7 ± 0.8 (139)+++
JRA4ATCC 9046,mucABCD 8.9 ± 0.7 (185)+++++
MLC2ATCC 9046, mucC 4.0 ± 0.3 (83)+++
MLC4ATCC 9046, mucCD 5.1 ± 0.2 (106)+++
  • a Alginate was determined in cells grown for 48 h on liquid Burke's nitrogen-free salts supplemented with 2% sucrose as a carbon source.

  • b + to +++++, least to most mucoidy, respectively.