Table 3.

Study of the expression of mutant fusion promoters inP. putida strain PaW85 and its ςS-deficient derivative PKS54

StrainPromoterMean β-Gal sp acta ± SD (wt/rpoS)b
4 h12 h24 h36 h48 h
WtL1Cla24.4 ± 1.9 (1.1)196.6 ± 20.1 (1.4)236.8 ± 8.3 (1.9)283.8 ± 14.8 (1.9)279.8 ± 8.9 (2.0)
rpoSL1Cla21.0 ± 0.5140.3 ± 7.2126.1 ± 3.2146.4 ± 4.3139.1 ± 0.4
WtL1-R15.1 ± 1.2 (0.8)62.5 ± 5.9 (2.2)168.3 ± 7.2 (3.6)287.2 ± 4.6 (4.8)202.1 ± 44.3 (2.4)
rpoSL1-R16.1 ± 1.328.3 ± 2.146.7 ± 5.459.7 ± 6.183.3 ± 15.2
WtL1-R25.8 ± 0.3 (0.6)53.9 ± 8.4 (1.0)94.1 ± 16.4 (0.9)153.2 ± 19.0 (1.2)123.8 ± 12.7 (1.1)
rpoSL1-R29.2 ± 1.055.1 ± 5.2109.3 ± 28.2130.4 ± 15.6112.9 ± 21.3
WtL1-R313.8 ± 2.9 (1.8)43.3 ± 1.2 (1.8)95.0 ± 1.8 (2.2)97.6 ± 2.7 (2.0)100.8 ± 3.9 (2.2)
rpoSL1-R37.8 ± 0.324.1 ± 1.743.5 ± 2.449.8 ± 4.146.1 ± 4.8
WtL1-R43.4 ± 0.6 (1.1)26.0 ± 0.8 (1.8)52.8 ± 2.9 (1.8)114.8 ± 15.3 (2.7)84.7 ± 4.8 (2.1)
rpoSL1-R43.0 ± 0.114.1 ± 2.729.7 ± 7.642.5 ± 9.939.6 ± 13.2
WtL1-R731.6 ± 1.6 (0.8)126.5 ± 7.9 (1.0)242.3 ± 29.0 (1.1)442.2 ± 13.3 (1.2)358.5 ± 15.8 (1.3)
rpoSL1-R738.1 ± 10.8122.7 ± 7.7222.3 ± 54.8364.2 ± 9.6282.2 ± 7.3
WtR1-L1118.4 ± 24.4 (1.2)874.3 ± 288.4 (1.3)2,358.4 ± 255.0 (0.9)1,417.9 ± 208.8 (1.4)1,344.3 ± 82.1 (1.4)
rpoSR1-L1100.6 ± 19.4659.5 ± 121.72,484.0 ± 274.8992.7 ± 65.0931.8 ± 107.3
WtR4-L1291.4 ± 27.3 (1.7)1,160.6 ± 101.9 (1.6)2,433.1 ± 161.0 (2.0)2,715.1 ± 116.9 (1.8)2,484.7 ± 120.0 (1.4)
rpoSR4-L1175.8 ± 9.8737.7 ± 59.11,214.2 ± 72.61,537.7 ± 152.71,825.9 ± 95.0
WtR7-L160.8 ± 2.5 (0.9)319.8 ± 22.0 (1.1)914.1 ± 69.3 (1.4)954.1 ± 42.1 (1.3)1,167.8 ± 99.0 (1.2)
rpoSR7-L166.2 ± 9.8286.7 ± 13.2647.1 ± 17.7714.4 ± 31.9987.7 ± 48.8
  • a β-Gal specific activity (nanomoles ofo-nitrophenol formed per minute per milligram of protein) was measured in P. putida PaW85 (wt) and PKS54 (rpoS) cells grown for 4, 12, 24, 36, and 48 h. Data of at least three independent experiments are presented.

  • b The effect of ςS on transcription from the promoters is expressed as the ratio of β-Gal activity in PaW85 cells to the β-Gal activity in PKS54 cells (wt/rpoS).