Table 1.

Strains and plasmids used

Strain or plasmidGenotypeSource (reference)
E. coli
 CS1562Fλ tolC::mini-Tn10 supE42 rph-1 C. Schnaitman (5)
 DPD1675 ilvB2101 ara thi Δ(proAB-lac) tolC::mini-Tn10 Lab strain (80)
 DPD1692 lacKanr Lab strain (17)
 DPD1718 lacKanr lacZ::recAφluxCDABE Lab strain (17)
 FB1ΔhisGDCBHAFI750 V. J. Davisson (12)
hisG2243 hisG2243 PEH, JHUa (30)
hisG3857 hisG3857 PEH, JHU (30)
hisD921 hisD921 PEH, JHU (30)
hisC901 hisC901 PEH, JHU (30)
hisC904 hisC904 PEH, JHU (30)
hisB463 hisB463 PEH, JHU (30)
hisH4744 F hisH4744 nadB29 thi mtl xyl ara lac PEH, JHU (31)
hisA323 hisA323 PEH, JHU (30)
hisF860 hisF860 PEH, JHU (30)
hisF891 hisF891 PEH, JHU (30)
hisI903 hisI903 PEH, JHU (30)
 MG1655F λ rph-1 D. Berg, Washington University (6)
 RFM443 rpsL galK2 lacΔ74 Rolf Menzel (56)
S. enterica serovar Typhimurium
 LT2 S. enterica serovar Typhimurium +K. Rudd, Miami
 TT7542 S. entericaserovar Typhimurium relA21::Tn10 K. Rudd
 TV101 S. enterica serovar Typhimuriumrfa Lab strain (81)
 LT2 relA S. enterica serovar TyphimuriumrelA21::Tn10 This study, P22 (TT7542) × LT2 → Tetr
Plasmids
 pBR322Cloning vector68
 pDEW326pUC18 + his′BHAF′ This work
 pDEW327pUC18 + his′BHAFI This work
 pDEW335pUC18 + his′GDCBHAFI′ This work
 phisAGIE-tac V. J. Davisson (21)
 pUC18Cloning vector68
 PVV101pBR322 +trpEDC′ C. Yanofsky, Stanford University
  • a PEH, JHU, Philip E. Hartman, Johns Hopkins University.