Table 2.

Primers

PrimerLength (mer)Sequence (5′–3′)a, location, and relevant propertiesReference or source
Used in fsrB deletion mutagenesis
 BDF131AAA GAG CTC GAA AGG GAT GAG TGA ACA AAT G, from bp −839 to −818 to start codon of fsrB, outer primer used for amplification offsrB 5′ regionThis study
 BDR131CCG AAT TCC TTT GTC CAT TGT GTT TTT CCT G, from bp 78 to 56 infsrB, inner primer used for amplification of fsrB5′ regionThis study
 BDF231CCG AAT TCT GGA TGG GAC AAA CTG AAA AAC A, from bp 54 to 23 before fsrB stop codon, inner primer used for amplification of fsrB 3′ regionThis study
 BDR231CCG GTA CCT AGC CAA CAA ACG AAT CAC AAC C, from bp 1020 to 999 after fsrBstop codon, outer primer used for amplification of fsrB 3′ regionThis study
Used for cotranscription study
 fsrARTF122ACA ATA CTT CTA ACG CTT TTG C, from bp 70 to 49 after fsrA start codonThis study
 orf1RTR120TAG TGG CGT GAC CTA CAT TG, from bp 28 to 9 before orf1 stop codonThis study
 fsrBRTF121GAT CTT CCT GAT CCA TAT CCA, from bp 52 to 32 after fsrB start codonThis study
 fsrARTR123CAA GGC ACT ATT TCT TAC TTA GG, from bp 22 before fsrA stop codon to bp 1 after fsrA stop codonThis study
 fsrCRTF121ACT CGT AAA AAG ACA AAA ACG, from bp 71 to 51 after fsrC start codonThis study
 fsrBRTR120TGA TGG TGT GGG AAC TAA GC, from bp 103 to 84 before fsrB stop codonThis study
 gelERTF121AGT ATT GCT TTA TCC TCC CTA, from bp 51 to 71 before gelE start codonThis study
 fsrCRTR121CTA AAA GTA ATC CAG AAG AGC, from bp 164 to 144 before fsrC stop codonThis study
 sprERTF122ATC AGA AAC AAA AAA CCA GCA C, from bp 74 to 53 after sprE start codonThis study
 gelERTR119CTC GTG ATG CGA TGC TTG C, from bp 148 to 130 before gelE stop codonThis study
Used for primer extension study
 APE122ACA ATA CTT CTA ACG CTT TTG C, from bp 70 to 49 after fsrA start codonThis study
 BPE221TAA GCC AGA AAA ATA AAC GGT, from bp 108 to 88 after fsrB start codonThis study
 EPE221CCA ACA AAG ATG CCT GTA CCT, from bp 50 to 30 aftergelE start codonThis study
Used for study of promoter activity
 APRF132CGG GAT CCT CTC AAG AAG ATA TAA AAT TAC AC, from bp −6 to −29 tofsrA start codon, used for amplification of fsrApromoterThis study
 APRR128CGG AAT TCG CGA CTC ATT CAA CAG GAA G, from bp −406 to −387 to fsrA start codon, used for amplification of fsrA promoterThis study
 BPRF129CGG GAT CCT CCT CTT CAA GTA TTG CAC TA, from bp −8 to −28 to fsrB start codon, used for amplification of fsrB promoterThis study
 BPRR130CGG AAT TCC AAG GCA CTA TTT CTT ACT TAG, from bp −110 to −89 to fsrB start codon, used for amplification of fsrB promoterThis study
 EPRF130CGG GAT CCT TCC CCA GTT TCC TTT TAT TTC, from bp −16 to −37 to gelE start codon, used for amplification of gelE promoterThis study
 EPRR129CGG AAT TCG CTA TGG TAT TGA GTT ATG AG, from bp −218 to −198 to gelE start codon, used for amplification of gelE promoterThis study
 BMP128CGG AAT TCA GGG AGG GAT AAT GAC TAA T, from bp −90 to −71 to fsrB start codonThis study
 BMP232CGG AAT TCA TTA AGG AAT TAT CTA TCT ATT AG, from bp −72 to −49 to fsrB start codonThis study
 BMP457CGG AAT TCG GGA TAA TGA CTA ATT AAG GAA TTA TCT ATC TAT TAG TCG CTA TAT TCG, from bp −85 to −37 tofsrB start codonThis study
 BMP563CGG AAT TCT AGG GAG GGA TAA TGA CTA ATA TTC CTT TTA TCT ATC TAT TAG TCG CTA TAT TCG, from bp −91 to −37 to fsrB start codon, bp −70 to −64 sequence TAA GGA A was changed to ATT CCT TThis study
 BMP662CGG AAT TCA GGG AAA AAT GTC GGC TGA TTA AGG AAT TAT CTA TCT ATT AGT CGC TAT ATT CG, from bp −188 to −170 to gelE start codon and from bp −72 to −37 tofsrB start codonThis study
 EMP127CGG AAT TCA GGG AAA AAT GTC GGC TGA, from bp −188 to −170 togelE start codonThis study
 EMP230CGG AAT TCA TTA AGG AAT TTA GAT AGT GCC, from bp −170 to −149 togelE start codonThis study
 EMP555CGG AAT TCA GGG AAA AAT GTC GGC TGA TTT TCC TTT TTA GAT AGT GCC GGT TAG G, from bp −188 to −142 to gelE start codon, bp −167 to −162 sequence AAG GAA was changed to TTC CTTThis study
 EMP656CGG AAT TCT AGG GAG GGA TAA TGA CTA ATT AAG GAA TTT AGA TAG TGC CGG TTA GG, from bp −91 to −73 tofsrB start codon and bp −170 to −142 to gelEstart codonThis study
 Vlac123GTT GAA TAA CAC TTA TTC CTA TC, flanking pTCV-lac cloning sites, used for sequencing inserts in pTCV-lac20
 Vlac221CTT CCA CAG TAG TTC ACC ACC, flanking pTCV-lac cloning sites, used for sequencing inserts in pTCV-lac20
Others
 lytF120ACA CCA ACC ACA GAA ACT AC, from bp 226 to 245 in E. faecalis autolysin geneThis study
 lytR120GGC AAT AAA TTC TGA AGG AC, from bp 555 to 536 in E. faecalis autolysin geneThis study
 gelEF121TGG TTG TGA TTC GTT TGT TGG, from bp −508 to −561 to gelE start codonThis study
 GBR222TGA CCA GAA CAG ATT CAC TTG G, from bp 9 to 30 before gelE stop codonThis study
  • a Linker sequences are underlined.