Table 2.

Plasmids used in this study

PlasmidRelevant propertiesaSource or reference
pBluescript II SK(+)Ampr, cloning vectorStratagene
pRK415Tcr, RK2-derived broad-host-range cloning vector 29
pBBR1MCSCmr, broad-host-range cloning vector 31
pKmobGIIKmr, mobilizable suicide vector 27
pCAM140AmprSm/Spr, contains 2.0-kb EcoRI fragment with Sm/Spr cassette and 2.0-kb NotI fragment with promoterless uidA gene 48
pMGmGmr, contains 1.9-kb Gmrcassette 35
pAS-LacZCmr, contains translational corS::lacZ fusion in pBBR1MCS, LacZ+ This study
pHL-PhoATcr, contains translationallscC::phoA fusion in pRK415, PhoA+ This study
pSKL3Ampr, containslscA on 3.0-kb PstI fragment (Plac>lscA), produces levan 23
pSKL3GAmpr, containslscA on a 3.0-kb PstI fragment (lscA>Plac), produces levan 23
pRA3.1Tcr, containslscA under control of Plac on 3.0-kbPstI fragment from pSKL3 in pRK415, produces levanThis study
pSKL3SmAmpr Sm/Spr, containslscA mutagenized by insertion of a Sm/Sprcassette in BstEII sites of pSKL3, levan deficient 23
pRA3.1-SmTcrSm/Spr, containslscA::Sm/Spr on 5.2-kb PstI fragment from pSKL3Sm in pRK415, levan deficientThis study
p7C7Tcr, genomic library clone of PG4180 with 25- to 30-kb insert in pRK7813, contains lscB, levan deficientThis study
pLB7.2Ampr, contains 7.2-kbEcoRV fragment from p7C7 in pBluescript (lscB>Plac), levan deficientThis study
pLB7.2RAmpr, contains 7.2-kb EcoRV fragment from p7C7 in pBluescript (Plac>lscB), levan deficientThis study
pRB7.2Cmr, carries lscB on 7.2-kbEcoRV fragment from pLB7.2 in pBBR1MCS, levan deficientThis study
pLB2.4Ampr, contains 2.4-kbPstI/SalI fragment from pLB7.2 derived by PCR in pBluescript (Plac>lscB), produces levanThis study
pLB7.2-GmAmpr Gmr, contains lscB mutagenized by insertion of Gmrcassette inserted in XhoI site of pLB7.2, levan deficientThis study
pKB7.2-GmKmrGmr, contains lscB mutagenized by insertion of Gmr cassette on 9.2-kb SalI/PstI fragment from pLB7.2-Gm in pKmobGII, levan deficientThis study
p5C10Tcr, genomic library clone of PG4180 with 25- to 30-kb insert in pRK7813, contains lscC, levan deficientThis study
pLC5.5Ampr, contains 5.5-kbPstI fragment from p5C10 in pBluescript (lscC>Plac), levan deficientThis study
pLC5.5-GmKmr Gmr, containslscC mutagenized by insertion of Gmr cassette inXhoI site of pLC5.5, levan deficientThis study
pKC5.5-GmKmr Gmr, containslscC::Gmr on 7.5-kb SalI fragment from pLC5.5-Gm in pKmobGII, levan deficientThis study
pLC5.5-SmAmpr Sm/Spr, containslscC mutagenized by insertion of Sm/Spr cassette in XhoI site of pLC5.5, levan deficientThis study
pKC5.5-SmKmr Sm/Spr containslscC::Sm/Spr on 7.5-kb SalI fragment from pLC5.5-Sm in pKmobGII, levan deficientThis study
  • a Sm/Spr, streptomycin and spectinomycin resistant; (Plac>lscA), the promoter of thelac operon directs transcription of lscA; (lscB>Plac), the promoter of thelac operon is located downstream of lscB.