Table 1.

B. subtilis strains, plasmids, and SPβ phage used

Strain, plasmid, or phageGenotype and phenotypeaSource and/or reference
B. subtilis strains
 AD52 cotD::cotD-lacZCmr Adam Driks (31)
 AD56 cotC::cotC-lacZCmr Adam Driks (31)
 PNZ-7SPβ::sspF-lacZCmr Scott Panzer (13, 25)
 780b trpC2 spoVFA::spoVFA-lacZ MLSr Jeff Errington (3)
 PS346 sspA::sspA-lacZCmr 8
 PS356ΔsspA ΔsspB αβ 9
 PS361ΔsspA ΔsspB sspA::sspA-lacZ αβ Cmr 8
 PS499ΔsspEγ 5
 PS533[pUB110] Kmr 29
 PS549[pUB-A] Kmr pUB-A→PS832
 PS578ΔsspA ΔsspB [pUB110] α βKmr 29
 PS579ΔsspA ΔsspB [pUB-A] α βKmr pUB-A→PS356
 PS832Wild-type derivative of strain 168Laboratory stock
 PS848SPβ::sspF-lacZMLSr PNZ-7→PS832
 PS850SPβ::sspF-lacZ ΔsspA ΔsspBα β MLSr PNZ-7→PS356
 PS1450ΔsspA ΔsspB [pSspCw t] α βKmr 29
 PS1485[pSspCw t] Kmr 29
 PS1612SPβ::sspF-lacZ[pSspCw t] KmrMLSr pSspCw t→PS848
 PS1613SPβ::sspF-lacZ[pSspCa l a] KmrMLSr pSspCa l a→PS848
 PS1614SPβ::sspF-lacZ ΔsspA ΔsspB[pSspCw t] α βKmr MLSr pSspCw t→PS850
 PS1615SPβ::sspF-lacZ ΔsspA ΔsspB[pSspCa l a] α βKmr MLSr pSspCa l a→PS850
 PS3196SPβ::spoIVCB-lacZCmr SPβ::spoIVCB-lacZ→PS832
 PS3197SPβ::spoIVCB-lacZ ΔsspA ΔsspBCmr SPβ::spoIVCB-lacZ→PS356
 PS3215 sspA::sspA-lacZ[pUB-A] Cmr Kmr pUB-A→PS346
 PS3216ΔsspA ΔsspB sspA::sspA-lacZ [pUB-A] αβ Cmr Kmr pUB-A→PS361
 PS3226 cotD::cotD-lacZ[pUB110] Cmr Kmr AD52→PS533
 PS3227 cotC::cotC-lacZ[pUB110] Cmr Kmr AD56→PS533
 PS3228 cotD::cotD-lacZ ΔsspA ΔsspB [pUB110] α βCmr Kmr AD52→PS578
 PS3229 cotC::cotC-lacZ ΔsspA ΔsspB [pUB110] α βCmr Kmr AD56→PS578
 PS3231 cotC::cotC-lacZ[pUB-A] Cmr Kmr AD56→PS549
 PS3233 cotC::cotC-lacZ ΔsspA ΔsspB [pUB-A] α βCmr Kmr AD56→PS579
 PS3236SPβ::spoIVCB-lacZ [pUB-A] Cmr Kmr pUB-A→PS3196
 PS3237SPβ::spoIVCB-lacZ ΔsspA ΔsspB [pUB-A] CmrKmr pUB-A→PS3197
 PS3276 cotC::cotC-lacZCmr AD56→PS832
 PS3277 cotC::cotC-lacZ ΔsspEγ Cmr AD56→PS499
 PS3355 spoVFA::spoVFA-lacZ[pUB110] Kmr MLSr 780→PS533
 PS3356 spoVFA::spoVFA-lacZ ΔsspA ΔsspB [pUB110] Kmr MLSr 780→PS578
Plasmids
 pSspCw t pUB110 with gene for SspCw t under sspB promoter control Kmr 29
 pSspCa l a pUB110 with gene for SspCa l aunder sspB promoter control Kmr 29
 pUB-ApUB110 with gene for SASP-α under control of its own promoter Kmr 8
Phage
 SPβ::spoIVCB-lacZ SPβ carryingspoIVCB-lacZ Cmr Irina Bagyan (7)
  • a MLS, erythromycin plus lincomycin.

  • b Note that the spoVFA gene is also called dpaA (3).