Table 2.

Effect of ompU and ompT mutations on virulence factor expression and/or secretion and antimicrobial resistance

Relevant genotypeaAutoagglutinationCT secretion (ng/ml of supernatant/OD600)TCP (CTX φ-Cm transductants/ml/OD600)Polymyxin B MIC (μg/ml)Serum resistance (no. of survivors relative to wild type/OD600)CT-B localization (% total)
CellsMedium
Wild type+5943.8 × 105 0.301.00.399.7
ΔompU1 17.85.9 × 104 0.150.972.997.1
ΔompU2 +3071.4 × 105 0.300.840.399.7
ΔompU2ΔompT +1041.1 × 105 0.301.361.198.9
ΔompT +2111.6 × 105 0.301.060.699.4
ΔtoxR 000.600.540.199.9
ΔtoxRΔompT 000.301.271.698.4
ΔepsD +43.82.7 × 105 NDb ND70.529.5
ΔepsE +NDb 1.5 × 105 NDND74.925.1
  • a The actual strains used (described in the text) were O395, KKV669, KKV780, KKV884, KKV809, KKV61, KKV804, KKV1216, and KKV1234.

  • b ND, not determined.