Table 1.

Bacterial strains and oligonucleotides used in this study

Strain or oligonucleotideGenotype or sequenceSource or reference
Strains
V. cholerae
  O395Classical O1, Ogawa, Smr 30
  KKV61ΔtoxR 24
  KKV598ΔlacZ 16
  KKV669ΔompU1 This study
  KKV780ΔompU2 This study
  KKV804ΔtoxR ΔompT This study
  KKV809ΔompT This study
  KKV884ΔompU2 ΔompT This study
  KKV1216ΔepsD::kan This study
  KKV1232ΔepsE::kanΔompU2 This study
  KKV1234ΔepsE::kan This study
E. coli
  DH5αF deoR endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1Δ(argF-lacZYA)U169 (φ80dlacZΔM15)17
  SM10λpir thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpirR6K; Kmr 31
Oligonucleotides
 OMPTP1HDIII5′-GGCAAGCTTAATCTTGAATGTTTTTTGTCAGCAT-3′
 OMPT1NDEI5′-AGTGGATTCATATGAAAAAACTCTATTAGCA-3′
 OMPT2SACII5′-TCCCCGCGGTTACCAGTAGATACGAGCGCCGATA-3′
 OMPTCTERM15′-TATGAGTATCATATGCAAGAAGCGAACACTGGC-3′
 OMPTCTERM25′-GCGGATCCATCGTTTTATCACCAAATAATCC-3′
 OMPTP2NDEI5′-GCGGATCCTCATATGAATCCACTGCCTTTTCTT-3′
 OMPU1BHI5′-GCGGATCCAATGATCAAGGTAAAAACGCGTC-3′
 OMPU1NDEI5′-GACTTAATCATATGAACAAGACTCTGATTGCT-3′
 OMPU2SACII5′-CGCCGCGGTTAGAAGTCGTAACGTAGACCGATAGC-3′
 OMPU2XBI5′-GCTCTAGACTCGTAGCCAGTGTAGTCAACGT-3′
 OMPUDEL15′-CAAACTAGGTCATATGGCGTTTACTGCGACATACAA-3′
 OMPUDEL25′-GCGCCGCGGAGATGAGTATTGAGTTCGAGA-3′
 OMPUP1ERI5′-GCGAATTCGTGGCACCAAAAATGACTTTGCC-3′
 OMPUP2BGLII5′-GCAGATCTAAGAGCAATCTGAGTCTTGTTCAT-3′
 OMPUP2NDEI5′-GCGGATCCTCATATGATTAAGTCCTAATTTATT-3′