Table 1.

Bacterial strains and oligonucleotides used in this study

Strain or oligonucleotideGenotype or sequenceSource or reference
Strains
V. cholerae
  O395Classical O1, Ogawa, Smr30
  KKV61ΔtoxR24
  KKV598ΔlacZ16
  KKV669ΔompU1This study
  KKV780ΔompU2This study
  KKV804ΔtoxR ΔompTThis study
  KKV809ΔompTThis study
  KKV884ΔompU2 ΔompTThis study
  KKV1216ΔepsD::kanThis study
  KKV1232ΔepsE::kanΔompU2This study
  KKV1234ΔepsE::kanThis study
E. coli
  DH5αF deoR endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1Δ(argF-lacZYA)U169 (φ80dlacZΔM15)17
  SM10λpirthi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpirR6K; Kmr31
Oligonucleotides
 OMPTP1HDIII5′-GGCAAGCTTAATCTTGAATGTTTTTTGTCAGCAT-3′
 OMPT1NDEI5′-AGTGGATTCATATGAAAAAACTCTATTAGCA-3′
 OMPT2SACII5′-TCCCCGCGGTTACCAGTAGATACGAGCGCCGATA-3′
 OMPTCTERM15′-TATGAGTATCATATGCAAGAAGCGAACACTGGC-3′
 OMPTCTERM25′-GCGGATCCATCGTTTTATCACCAAATAATCC-3′
 OMPTP2NDEI5′-GCGGATCCTCATATGAATCCACTGCCTTTTCTT-3′
 OMPU1BHI5′-GCGGATCCAATGATCAAGGTAAAAACGCGTC-3′
 OMPU1NDEI5′-GACTTAATCATATGAACAAGACTCTGATTGCT-3′
 OMPU2SACII5′-CGCCGCGGTTAGAAGTCGTAACGTAGACCGATAGC-3′
 OMPU2XBI5′-GCTCTAGACTCGTAGCCAGTGTAGTCAACGT-3′
 OMPUDEL15′-CAAACTAGGTCATATGGCGTTTACTGCGACATACAA-3′
 OMPUDEL25′-GCGCCGCGGAGATGAGTATTGAGTTCGAGA-3′
 OMPUP1ERI5′-GCGAATTCGTGGCACCAAAAATGACTTTGCC-3′
 OMPUP2BGLII5′-GCAGATCTAAGAGCAATCTGAGTCTTGTTCAT-3′
 OMPUP2NDEI5′-GCGGATCCTCATATGATTAAGTCCTAATTTATT-3′