Table 1.

PCR and sequencing primers used in this study

PrimerFunctionPositionOrientationBacterial specificitySequence
D88PCR7–27ForwardMostGAGAGTTTGATYMTGGCTCAG
E94PCR1525–1541ReverseMostGAAGGAGGTGWTCCARCCGCA
C90PCR1483–1503Reverse Spirochaetes GTTACGACTTCACCCTCCT
F01PCR1487–1508Reverse Bacteroidetes CCTTGTTACGACTTAGCCC
C75Sequencing7–27ForwardMostGAGAGTTTGATYCTGGCTCAG
B34Sequencing344–358ForwardMostACGGGAGGCAGCAGY
F16Sequencing789–806ForwardMostTAGATACCCYGGTAGTCC
F18Sequencing1099–1113ForwardMostGCAACGAGCGCAACC
F19Sequencing1099–1114Forward Bacteroidetes ATAACGAGCGCAACCC
E94Sequencing1522–1541ReverseMostGAAGGAGGTGWTCCARCCGCA
F20Sequencing1226–1242ReverseMostCCATTGTARCACGTGTG
F21Sequencing1226–1242Reverseβ-Proteobacteria CCATTGTATGACGTGTG
F17Sequencing907–926ReverseMostCCGTCWATTCMTTTGAGTTT
F15Sequencing519–533ReverseMostTTACCGCGGCTGCTG
F22Sequencing344–358ReverseMostRCTGCTGCCTCCCGT