Table 3.

Expression of virBp::lacZin an isogenic E. coli crp mutant background

StrainU of β-galactosidase activity ina:
IM plus glucoseIM plus glycerol
−AS+AS−AS+AS
M182(pSL204/pPS1.3)2.230.21.465.2
M182(pSL204/pPS1.3R)1.13.65.16.4
M182Δcrp(pSL204/pPS1.3)01.12.30.1
M182Δcrp(pSL204/pPS1.3R)2.23.42.12.9
  • a Cultures were grown for 18 h in induction medium at pH 6.0 (IM) containing 55 mM glucose or glycerol. Acetosyringone (AS) was added where indicated to a final concentration of 200 μM. Values are averages from three replicates and are expressed as units of β-galactosidase activity.