Table 1.

Bacterial strains and plasmids

Strain or plasmidGenotype or phenotypeSource or reference
E. coli
 DH5α recA endA1 hsdR17 supE4 gyrA96 relA1Δ(lacZYA-argF)U169(φ80dlacZΔM15)41
 MC4100F araD139Δ(argF-lac)U169 rpsL150 relA flb5301 ptsF25 deoC1 7
 M182K-12 Δlac crp + 8
 M182Δcrp K-12 Δlac Δcrp 4
Plasmids
 pIB410 virBp::lacZIncP Tcr 5
 pPC401 lacp::virG fusion in pTZ18R, Apr 25
 pSW191 lacp::virA fusion in pTZ18R, Apr 54
 pSG692 lacp::virA andlacp::virG in pTZ18R, Apr This work
 pSY215 virB::lacZ Plac::virG(N54D) IncW Gmr 31
 pSL107 lacp::virA lacp::virG virBp::lacZColE1 IncP Apr Tcr 31
 pSL204 lacp::virA lacp::virG virBp::lacZIncP Tcr This work
 pSJ0101 lacp::virA virBp::lacZ IncP Tcr This work
 pSJ0102 lacp::virG virBp::lacZ IncP Tcr This work
 pTZ18R/pTZ19RCloning vectors, ColE1 Apr U.S. Biochemicals
 pTC110Cloning vector, IncW KmrSpr 10
 pPS1.3 lacp::rpoA fromA. tumefaciens A136 in pTZ19R, Apr 31
 pPS1.3RNon-lacp-driven rpoA fromA. tumefaciens A136 in pTZ18R, Apr 31
 pHO98 lacp::rpoA from A. tumefaciens A136 in pTC110, Kmr IncW31
 pTC1.1 lacp::chvE fromA. tumefaciens A136 in PCR2.1-TOPO, Apr This work
 pTCR2.4 lacp::chvE, lacp::rpoA from A. tumefaciensA136 in PCR2.1-TOPO, Apr This work
 pQE30 and pQE31His expression vector, Apr Qiagen
 pZL2 A. tumefaciens His-RpoA, Apr 31
 pECH E. coli His-RpoA, Apr 31
 pAD8 A. tumefaciens His-RpoA (deletion of C-terminal eight amino acids), Apr This work
 pEA8 E. coli His-RpoA (1–329 plus A. tumefaciens RpoA C-terminal eight amino acids), Apr This work
 pADC A. tumefaciensHis-RpoA (1–242 amino acids), Apr This work
 pEN E. coli His-RpoA (1–247 amino acids), Apr This work
 pENACNHis-RpoA (E. coli 1–247 amino acids plus A. tumefaciens 248–336), Apr This work
 pANECHis-RpoA (A. tumefaciens 1–242 amino acids plus E. coli243–329)This work