Table 2.

Survival of P. putida DOT-T1E and its mutant derivatives upon sudden solvent shock

StrainNonfunctional Ttg pump(s)Cell survivala with:
ToluenePropylbenzeneEthylbenzenem-XyleneStyrene
NIbIbNIINIINIINII
DOT-T1E10−4–10−5 0.5–1 1 110−2 10−2  1 110−2  1
DOT-T1E-18ABC<10−8 10−3–10−4  1 110−2 10−2 10−3  110−7 10−2
DOT-T1E-1DEF10−4–10−5 10−2–10−3  1 110−2 10−2  1 110−2 10−2
DOT-T1E-PS28GHI<10−8 10−7  1 110−2 10−2  1 110−7 10−6
DOT-T1E-82ABC, DEF<10−8 10−5  1 110−2 10−2 10−3  110−7 10−5
DOT-T1E-PS30DEF, GHI<10−8 <10−8  1 110−2 10−2  1 1<10−8 10−7
DOT-T1E-PS32ABC, GHI<10−8 <10−8 10−4 10−3 10−4–10−5 10−4 10−4–10−5 10−4 <10−8 <10−8
DOT-T1E-PS34ABC, DEF, GHI<10−8 NVc 10−4–10−5 NV10−5 NV10−5 NV<10−8 NV
  • a Survival refers to the fraction of viable cells 10 min after the addition of 0.3% (vol/vol) solvent to cultures grown in LB medium (noninduced) or in LB medium with toluene supplied through the gas phase (induced).

  • b NI, noninduced cells; I, induced cells.

  • c NV, nonviable cells; the assay was not performed.