Table 3.

Complementation analysis oftraG::lacZ induction in mutant NTM7 with two genomic cosmids and their subclones

StrainComplementing cosmid or cloneβ-Galactosidase activity fromtraG::lacZa% of complementationb
NT1pRK415GIII94NAc
NTM7pRK415GIII<1NA
NTM7pZQL98388
NTM7pZQL108590
NTM7pZLR112830
NTM7pZLG73032
NTM7pZLP1622021
NTM7pZLP1632324
NTM7pZLD1444
  • a β-Galactosidase activity expressed from pDCKI41 present in each mutant was analyzed as described in Materials and Methods (expressed as units per 109 CFU). No significant difference was observed when exogenous 3-oxo-C8-HSL was provided at a final concentration of 25 nM. Similar results were obtained in three independent experiments.

  • b Calculated relative to the level of β-galactosidase activity present in NT1(pDCKI41, pRK415GIII).

  • c NA, not applicable.