Table 6.

A mutation in traM partially restores the expression of the traG::lacZ reporter fusion in NTM7

Test strainrnd genotypeReporter Ti plasmidtraM genotypeβ-Galactosidase activitya
NT1+pDCKI41+92
pKMI41b 114
NTM7pDCKI4+<1
pKMI41b 46
  • a β-Galactosidase activity, assayed as described in Materials and Methods, is expressed as units per 109 CFU.

  • b pKMI41 is a derivative of pDCKI41 with annptII insertion in traM (32).