Table 1.

Strains, plasmids, and primers used in this study

Strain, plasmid, or primerRelevant characteristicsRelevant mutation(s)ReporterReference, source, or derivation
Strains
B. subtilis
  CU1065W168attSPβtrpC2 37
  ZB307AW168 SPβ2Δ2::Tn917::pBSK10Δ640
  BFS1816168 wild-typeohrA-lacZ ohrA ohrA 38
  BFS1818168 wild-typeohrB-lacZ ohrB ohrB 38
  HB574CU1065ohrA-lacZ ohrA ohrA This work
  HB575CU1065ohrB-lacZ ohrB ohrB This work
  HB1703CU1065 ahpC::Tn10(1603) ahpC 7
  HB2000CU1065ohrR::kan ohrR This work
  HB2001HB574ohrR::kan ohrR, ohrA ohrA This work
  HB2002HB575ohrR::kan ohrR, ohrB ohrB This work
  HB2003HB574ohrB::pGEMCAT ohrA, ohrB ohrA This work
  HB2006ZB307A SPβc2Δ2::Tn917::φ(ohrR′-cat-lacZ) ohrR This work
  HB2007ZB307A SPβc2Δ2::Tn917::φ(ohrA′-cat-lacZ) ohrA This work
  HB2008HB574ahpC::Tn10 ohrA, ahpC ohrA This work
  HB2009HB575ahpC::Tn10 ohrB, ahpC ohrB This work
  HB2010HB2003ahpC::Tn10 ohrA, ohrB, ahpC ohrA This work
  HB2011CU1065 SPβc2Δ2::Tn917::φ(ohrR′-cat-lacZ) ohrR This work
  HB2012CU1065 SPβc2Δ2::Tn917::φ(ohrA′-cat-lacZ) ohrA This work
  HB2013HB2000 SPβc2Δ2::Tn917::φ(ohrR′-cat-lacZ) ohrR ohrR This work
  HB2014HB2000 SPβc2Δ2::Tn917::φ(ohrA′-cat-lacZ) ohrR ohrA This work
  HB2031CU1065 SPβc2Δ2::Tn917::φ(ohrA-cat-lacZ) ohrA This work
  HB2044HB2000 SPβc2Δ2::Tn917::φ(ohrA-cat-lacZ) ohrR ohrA This work
  HB6506HB1000ahpC::Tn10(1603) ahpC 7
E. coli
  DH5αφ80lacZΔM15recA1 endA1 gyrA96 thi-1 hsdR17(rK , mK +)supE44 relA1 deoRΔ(lacZYA-argF)U169Lab stock
  GM 2163F ara-14 leuB6 thi-1 fhuA31 lacY1 tsx-78 galK2 galT22 supE44 hisG4 rpsL1 (Strr) xyl-5 mtl-1 dam13::Tn9 (Cmr)dcm-6 mcrB1 hsdR2 (rK , mK +) mcrA NEB
Plasmids
 pGEM-catpGEM-3zf(+)-cat-1 (carrying Cmr gene)39
 pGEM-mApGEM-3zf(+) with PstI-SphI containing ohrA′-ohrR-ohrB This work
 pBC-zApBCSK (Stratagene) containingohrB This work
 pJPM122 cat-lacZ operon fusion vector for SPβ34
 pDG792pMTL23 containing Kanrcassette19
 pMF1pGEM-mA containing theBamHI-BglII Kanr cassette (1.6 kb) from pDG792 at BclI site inohrR ohrR This work
 pMF2pGEM-cat containing intergenicSphI-EcoRI fragment ofohrB This work
 pMF3pJPM122 withohrA promoterThis work
 pMF4pJPM122 with ohrR promoterThis work
Primers
 3665′-ACTCTCCGTCGCTATTGTAACCAG-3′Lab stock
 495 (forward)5′-CGGGATCCTAGCGGGGTAATGTTCAATG-3′This work
 496 (reverse)5′-CCGAATTCAAAAGCGGTTGACATTCCAG-3′This work
 497 (forward)5′-CGGGATCCTGTATTGCTTTGTCATCTCC-3′This work
 519 (reverse)5′-CGGGATCCAAATCAAGAACACCGTCATC-3′This work
 527 (forward)5′-GGTGAACACCATGGAAAATAAATT-3′This work
 528 (reverse)5′-CCGGATCCGTTGCTGAATAAATAAA-3′This work
 529 (reverse)5′-CGGGATCCAATGACCTTTCCTTCTCTTC-3′This work
 530 (reverse)5′-CCCAAGCTTAAATCAAGAACACCGTCATC-3′This work
 531 (forward)5′-CGGGATCCTATATTGGGGGAATGAAAAA-3′This work
 5355′-GTACATATTGTCGTTAGAAC-3′This work
 536 (reverse)5′-AATGTCAACCGCTTTTTCT-3′This work
 PE5′-AACGCGGTCTGATCAAATGA-3′This work